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Coherence Controlled Holographic Microscope with the digital optics
Vavřinová, Jana ; Jákl, Petr (referee) ; Dostál, Zbyněk (advisor)
The Digital Micromirror Device (DMD) technology has been developed especially for Digital Light Processing projectors, which allow the image projection. After this succesful implementation, and thanks to the commercial availibility and low initial cost of the DMD chip, a wide range of other applications became possible. Besides, it may be used in microscopy as a spatial light modulator. For example in Coherence-Controlled Holographic Microscope (CCHM) that finds its use especially for imaging and measurement of live-cell dynamic processes. The DMD chip placed in the illumination part of CCHM allows for broadening the application possibilities. Namely it could be different illumination mode experiments or tomographic applications. The master's thesis deals with the optical design of CCHM with digital optics, i. e. DMD chip. The selection of optical elements for CCHM, the experimental verification of the imaging setup and the process of designing the illumination part are described in detail. In the end, the analysis of different designs for illumination setup with the digital optics in object arm is carried out and the results are compared.
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Phase imaging below the diffraction limit
Nečesal, Daniel ; Bouchal, Zdeněk (referee) ; Dvořák, Petr (advisor)
Tato diplomová práce se zabývá konstrukcí interferenčních zařízení pro výzkum měření fáze světla na mikro-strukturách a fáze plazmonů na nano-strukturách. V první kapitole je vybudován teoretický základ pro optiku a nano-fotoniku používané v budoucích kapitolách. Následně je popsána interference vln a jejich praktické použití pro prolomení difrakčního limitu pomocí holografie plazmonů. Prvním experimentální sestavou je Machův-Zehnderův holografický mikroskop. Je popsán způsob, jak ho sestavit z běžně dostupných součástek a jak je navržen ovládací software k jeho používání. Následně jsou popsány výsledky naměřené pomocí tohoto zařízení. V poslední kapitole se zaměříme na sestavení holografického SNOM přístroje pro studování plazmonů a jejich interference. Nejdříve popíšeme základní princip a navržení softwaru pro automatizaci měření, pro zrychlení vědeckých postupů. Nakonec předložíme výsledky měření mikroskopu.
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Polarization Imperfections of Light in Interferometry
Kučera, Petr ; Lazar, Josef (referee) ; Mohr, Fridemann (referee) ; Wilfert, Otakar (advisor)
Disertační práce pojednává o polarizačních nedokonalostech optických komponentů, které jsou využívány ke kontrole a k transformaci polarizačního stavu světla. Získané teoretické výsledky jsou pak využity ve vybraných aplikacích, jež ke své činnosti využívají právě polarizace světla. Konkrétně se jedná o zařízení měřící vibrace oscilujících objektů, dále o interferenční měření dvojlomu v transparentních materiálech a konečně, o vybraná témata z optické kvantové komunikace.
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Review of methods to determining wear in hip replacements
Zapletal, Jakub ; Vrbka, Martin (referee) ; Ranuša, Matúš (advisor)
This bachelor thesis analysis issues of observation and measurement of wear on hip replacements. First of all there is a description of historical milestones in development of hip prosthesis. Afterwards there is a recherché study of measuring methods and devices, whereas each one of them is reviewed for pros and cons. In the last section these methods are compared and there is an author’s recommendation for the most viable method for observation and measurement of wear on hip prosthesis.
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Fiber guided illumination module
Kropáč, Vlastimil ; Kvasnica, Lukáš (referee) ; Dostál, Zbyněk (advisor)
This diploma thesis describes the design of the illumination system for a Coherence--Controlled Holographic Microscope (CCHM). The theoretical part mentions the history of microscopy, the principle of holography and individual types of interference microscopy. To get closer to the topic, individual light sources and an overview of current illumination systems are mentioned. The diploma thesis also describes the procedure of designing a fiber-optic illumination module for microscopy from optical design through design of construction to the last step, which is assembly and testing of the module.
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Application possibilities of refractometer for liquids diagnostics
Macháč, Milan ; Kazelle, Jiří (referee) ; Frk, Martin (advisor)
This bachelor thesis focuses on application possibilities of refractometer for liquids diagnostics. The first part of the thesis focuses on the theoretical basics propagation of electromagnetic waves. The next section focuses on the measurement of refractive index, refractometry, a description of each type refractometers and short introduction to interferometry. The penultimate chapter is devoted to an application using a refractometer and what can measure and calculate. The last chapter is a practical part, there is described a calibration process, the measured values of the refractive index of liquids and evaluate the results.
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Automated Procedures for Coherence Controlled Holographic Microscope
Dostál, Zbyněk ; Štarha, Pavel (referee) ; Jákl, Petr (referee) ; Chmelík, Radim (advisor)
Coherence-Controlled Holographic Microscope (CCHM) and a Fluorescence Holographic Microscope (FHM) were developed particularly for quantitative phase imaging and measurement of live cell dynamics, which used to be a subject of digital holographic microscopy (DHM). CCHM and FHM in low-coherence mode extend capabilities of DHM in the study of living cells. However, this advantage following from the use of low coherence is accompanied by increased sensitivity of the system to its correct alignment. Therefore, the introduction of an automatic self-correcting system is inevitable. Accordingly, in the thesis, the theory of a suitable control system is derived and the design of an automated alignment system for both microscopes is proposed and experimentally proved. The holographic signal was identified as a significant variable for guiding the alignment procedures. On this basis the original basic realignment algorithms were proposed, which encompasses the processes for initial and advanced alignment as well as for long-term maintenance of the microscope aligned state. Automated procedures were implemented in both microscopes unique set of robotic mechanisms designed and built within the frame of the thesis work. All of the procedures described in the thesis were in real experimentally proved at real microscopes in the experimental biophotonics laboratory. In addition, the control software, which contains the needed automated procedures, was developed for FHM.
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Distributed fiber optic sensors
Holík, Tomáš ; Čučka, Milan (referee) ; Münster, Petr (advisor)
The thesis deals with the possibility of using optical fiber as a sensor. The introduction of the thesis is devoted to the foundations of optics. In addition, the thesis deals with optical interferometry, polarization and dispersion in optical fiber. The main output of the thesis is the comparison of acoustic vibration measurements using interferometric and polarization methods and further measurements on the real route in Brno.
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Workstation for optical interferometry
Blecha, Martin ; Honec, Peter (referee) ; Janáková, Ilona (advisor)
This thesis is specialized on composition of workplace for experiments flowing from optical interferometry. Here are described laboratory exercise servant for demonstration basic principles of interferometry. In more details is described method measurement of height profile objects with method white light interferometry.
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